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KMID : 0387720060170010071
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Korean Journal of Blood Transfusion
2006 Volume.17 No. 1 p.71 ~ p.61
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Detection of an AB(weak) Blood Group by Genotyping.
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Chung Hye-Yoon
Yoon Mi-Sook
Kim Young-Jin
Kim Dong-Chan
Park Sung-Sup
Han Kyou-Sup
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Abstract
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Since precise ABO blood group typing is the most important part of a safe blood transfusion, the cause of an ABO discrepancy should be determined before issuing blood. The authors confirmed the ABO blood group of a 25-year-healthy male using genotyping method in those who showed an ABO blood group discrepancy between the cell and serum types. ABO typing, saliva test, adsorption elution test and serum transferase assay were performed and the presence of a weak B substance and B transferase was suspected without any evidence of the B antigen on red blood cells. Polymerase-chain-reaction restriction-fragment-length-polymorphism (PCR-RFLP) analysis was performed using the restriction enzymes including BstE II, Pvu II, BssH II, Alu I, and Mva I. The genotyping result showed a normal A allele and B allele. DNA analysis of exons 6 and 7 revealed normal A and B sequences. Therefore, we confirmed A(1)B(weak) blood type.
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KEYWORD
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ABO blood group, Genotyping, PCR-RFLP
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